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Fig. 3 | Virology Journal

Fig. 3

From: Two mutations in the HR2 region of Newcastle disease virus fusion protein with a cleavage motif “RRQRRL” are critical for fusogenic activity

Fig. 3

Identification of specific amino acids involved in fusogenic activity. a Syncytium formation in BHK-21 cells co-transfected with La-F* and La-HN. b Quantitative analysis of the syncytia in (a). Statistical significance is designated with lowercase letters (P < 0.05) or capital letters (P < 0.01). c Proteolytic cleavage of the F* proteins in transfected BHK-21 cells was analyzed by Western blot. GAPDH was used as a control. d Surface expression of the F* proteins in transfected BHK-21 cells by flow cytometry analysis

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