Recombinant expression of beak and feather disease virus capsid protein and assembly of virus-like particles in Nicotiana benthamiana

Table 1 Primers for PCR amplification used during molecular cloning

Product		Sense primer		Antisense primer
Description	Size (bp)	Sequence (5′ - 3′)	Cloning site	Sequence (5′ - 3′)	Cloning site
BFDV BKS1ZA_84 cp 6xHis		GTAACGCGTTAGGTACATGTGGGGCACCTCTAAC	AflIII/MluI	GTGATGGTGATGCCCTTCAGTTCTGGGATTATTGG
BFDV BKS1ZA_84 cp 6xHis	796	GTAACGCGTTAGGTACATGTGGGGCACCTCTAAC	AflIII/MluI	CATCTCGAGCTAGTGATGGTGATGGTGATGCCCTTC	XhoI
rbcs1-cTP BFDV BKS1ZA_84 cp 6xHis	959	GGACCATGGCTTCCTCTGTTATTTCCTC	NcoI	CATCTCGAGCTAGTGATGGTGATGGTGATGCCCTTC	XhoI
BFDV BKS1ZA_84 cp 6xHis SEKDEL		GTAACGCGTTAGGTACATGTGGGGCACCTCTAAC	AflIII/MluI	CTCATCTTTCTCAGAGTGATGGTGATGGTGATGCC
BFDV BKS1ZA_84 cp 6xHis SEKDEL	814	GTAACGCGTTAGGTACATGTGGGGCACCTCTAAC	AflIII/MluI	CCTCTCGAGCTAGAGCTCATCTTTCTCAGAGTGAT	XhoI
LPH BFDV BKS1ZA_84 cp 6xHis SEKDEL	860	GGACCATGGAGTGGAGCTGGATCTTC	NcoI	CCTCTCGAGCTAGAGCTCATCTTTCTCAGAGTGAT	XhoI
LPH BFDV BKS1ZA_84 cp 6xHis	840	GGACCATGGAGTGGAGCTGGATCTTC	NcoI	CATCTCGAGCTAGTGATGGTGATGGTGATGCCCTTC	XhoI

ISSN: 1743-422X