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Table 2 RT-PCR primers used for sequencing the complete genome (A) and for a novel qRT-PCR assay to quantify virus load (B)

From: Isolation and characterization of the fall Chinook aquareovirus

A.
 VP1-F TGT GAC ATC GGC GAC AAC
 VP1-R CTG ACG TGA CCA TGC CAA T
 VP2-F TGG ATG CCC CTA GAC TTT G
 VP2-R TGT CCG AAT TAT GAC AAT AGT C
 VP3-F CCG ATG GTC AGC TCT CG
 VP3-F-outer CAC CCA GAA CAA CAA TGG
 VP3-R GGG GAC TTT GTC TCC GA
 VP4-F TAC TTC CCG TGA CGT CC
 VP4-R TTG AAG CTT GAA CCA AGC GG
 VP5-F CCG CAA TTG GTA CCA CTG
 VP5-R CAG CGG TTC CCA TGA GT
 VP6-F GCG ATG GAA CTC GCT C
 VP6-R GCA TTA AGA TGG GCA CCG
 NS2-F TGA TGT GGC AGA AGC CTG
 NS2-R TGG CGC CAT TGA GAC C
 VP7-F TCT ACG AGC AAT CGC GC
 VP7-R ATA CCT GGT ACT GTC CAG
 NS3-F GTT AAA TGG TAC GGC GGC
 NS3-R TAT GGG TCT GCA GCG TG
 NS4-F GCC TGG TTC GAC TAC ATC
 NS4-R GCA CGT CCA ACC TTC TTG
 NS1–2-F TAC GGC CAA GGT CAG TTC
 NS1–2-R GGT TGG GAA TGA AGC GAA GA
 NS1–1-F CAC AGC TCG GCG TCA C
 NS1–1-R TAA GTG ATT GTC CAG GTG G
B.
 qVP2-F GGC GTA ATC CAG CCG C
 qVP2-R GCT AGT GAA GGG ATC GTC