Table 2 RT-PCR primers used for sequencing the complete genome (A) and for a novel qRT-PCR assay to quantify virus load (B)
From: Isolation and characterization of the fall Chinook aquareovirus
A. | |
VP1-F | TGT GAC ATC GGC GAC AAC |
VP1-R | CTG ACG TGA CCA TGC CAA T |
VP2-F | TGG ATG CCC CTA GAC TTT G |
VP2-R | TGT CCG AAT TAT GAC AAT AGT C |
VP3-F | CCG ATG GTC AGC TCT CG |
VP3-F-outer | CAC CCA GAA CAA CAA TGG |
VP3-R | GGG GAC TTT GTC TCC GA |
VP4-F | TAC TTC CCG TGA CGT CC |
VP4-R | TTG AAG CTT GAA CCA AGC GG |
VP5-F | CCG CAA TTG GTA CCA CTG |
VP5-R | CAG CGG TTC CCA TGA GT |
VP6-F | GCG ATG GAA CTC GCT C |
VP6-R | GCA TTA AGA TGG GCA CCG |
NS2-F | TGA TGT GGC AGA AGC CTG |
NS2-R | TGG CGC CAT TGA GAC C |
VP7-F | TCT ACG AGC AAT CGC GC |
VP7-R | ATA CCT GGT ACT GTC CAG |
NS3-F | GTT AAA TGG TAC GGC GGC |
NS3-R | TAT GGG TCT GCA GCG TG |
NS4-F | GCC TGG TTC GAC TAC ATC |
NS4-R | GCA CGT CCA ACC TTC TTG |
NS1–2-F | TAC GGC CAA GGT CAG TTC |
NS1–2-R | GGT TGG GAA TGA AGC GAA GA |
NS1–1-F | CAC AGC TCG GCG TCA C |
NS1–1-R | TAA GTG ATT GTC CAG GTG G |
B. | |
qVP2-F | GGC GTA ATC CAG CCG C |
qVP2-R | GCT AGT GAA GGG ATC GTC |