Table 1 Pan-specific degenerate RT-PCR primers used for (A) identification of isolates of Aquareovirus species A or B in an attempt to identify the fall Chinook aquareovirus (Batts, unpublished data). (B) Modified primers for identification of isolates of species Aquareovirus B based upon sequence analysis that includes the fall Chinook aquareovirus
From: Isolation and characterization of the fall Chinook aquareovirus
A. | |
Aquareovirus A | |
1811F | C RCC ATG GAG ACC AAA CC |
1812R | CT STG RTT CAT CAT AGC GTG |
1813F | AY ATC ACY CAY SAG TGT CA |
1814R | GC TAG ATC TTT GCC ATA GAA |
Aquareovirus B | |
1807F | C GCC ATG GAT ACC AAG CC |
1809R | TG GGA CAG CAG GGC GTG |
1808F | AY WWC MCT CAC GAG TGT CA |
1810R | GM KAG ATC CTT GCC GTA RAA |
B. | |
4370F | ACC ATC GCY ATG GAT AYY AAG CC |
4371R | CG GTT SGA SCC ATG ACG RTT CTC |
4372F | ACC GTW GCC AAC GCA CTT TGC GA |
4373R | G CGC GAT TGS WSS TAG AGA GGA |