Skip to main content

Table 1 Details of the tested vaccine mixtures

From: Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus

Animal model

Group

PTAI compositions or Lipofectin

Molar ratio PTAI: helper lipids

DNA dose

Ratioa

Chicken-broilers

1

PTAI-6-8 with DOPE

1.5:1

125 μg

0.9:1

2

PTAI-11 with DOPE/DC

1:1:1

125 μg

0.8:1

3

PTAI-11 with DOPE/DC/DOPC

1:1:1:1

125 μg

1.3:1

4

PTAI-15 with DOPE/DC/DOPC

1:1:1:1

125 μg

1.6:1

5

Lipofectin

n.a

125 μg

1:6

6

PTAI-6-8 with DOPE or Lipofectinb

1.5:1

125 μg

0.9:1 or 1:6

Chicken-layers

1

PTAI-11 with DOPE/DC

1:1:1

125 μg

0.8:1

2

PTAI-11 with DOPE/DC

1:1:1

62.5 μg

1.6:1

3

PTAI-11 with DOPE/DC

1:1:1

62.5 μg

0.8:1

4

Lipofectin

n.a

125 μg

1:6

5

Lipofectin

n.a

62.5 μg

1:6

6

None

n.a

125 μg

n.a

7

None

n.a

62.5 μg

n.a

8

Lipofectin

n.a

125 μg

1:6

Mice

1

Lipofectin

n.a

10 μg

1:6

2

PTAI-10-14 with DOPE/DC

1:1:1

10 μg

0.8:1

3

PTAI-10-14 with DOPE/DC

1:1:1

10 μg

0.8:1

  1. Vaccine mixtures were prepared by mixing the K3 plasmid with PTAI compositions or Lipofectin. Groups 6 and 7 (chicken-layers) were immunized with vaccine containing the K3 plasmid in PBS, pH 7.4 without any carriers. aratio for PTAI:DNA (w:w) or for Lipofectin:DNA (v:w) in vaccine doses is indicated; bin control group empty pCI vector was used with PTAI-6-8 (n = 3) or Lipofectin (n = 2); n.a not applicable
Back to article page

Virology Journal

ISSN: 1743-422X