Skip to main content

Advertisement

Table 1 Details of the tested vaccine mixtures

From: Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus

Animal model Group PTAI compositions or Lipofectin Molar ratio PTAI: helper lipids DNA dose Ratioa
Chicken-broilers 1 PTAI-6-8 with DOPE 1.5:1 125 μg 0.9:1
2 PTAI-11 with DOPE/DC 1:1:1 125 μg 0.8:1
3 PTAI-11 with DOPE/DC/DOPC 1:1:1:1 125 μg 1.3:1
4 PTAI-15 with DOPE/DC/DOPC 1:1:1:1 125 μg 1.6:1
5 Lipofectin n.a 125 μg 1:6
6 PTAI-6-8 with DOPE or Lipofectinb 1.5:1 125 μg 0.9:1 or 1:6
Chicken-layers 1 PTAI-11 with DOPE/DC 1:1:1 125 μg 0.8:1
2 PTAI-11 with DOPE/DC 1:1:1 62.5 μg 1.6:1
3 PTAI-11 with DOPE/DC 1:1:1 62.5 μg 0.8:1
4 Lipofectin n.a 125 μg 1:6
5 Lipofectin n.a 62.5 μg 1:6
6 None n.a 125 μg n.a
7 None n.a 62.5 μg n.a
8 Lipofectin n.a 125 μg 1:6
Mice 1 Lipofectin n.a 10 μg 1:6
2 PTAI-10-14 with DOPE/DC 1:1:1 10 μg 0.8:1
3 PTAI-10-14 with DOPE/DC 1:1:1 10 μg 0.8:1
  1. Vaccine mixtures were prepared by mixing the K3 plasmid with PTAI compositions or Lipofectin. Groups 6 and 7 (chicken-layers) were immunized with vaccine containing the K3 plasmid in PBS, pH 7.4 without any carriers. aratio for PTAI:DNA (w:w) or for Lipofectin:DNA (v:w) in vaccine doses is indicated; bin control group empty pCI vector was used with PTAI-6-8 (n = 3) or Lipofectin (n = 2); n.a not applicable