Fig. 3

Comparison of humoral response of layers after immunization with different K3 vaccine mixtures. Vaccine doses composed of K3 plasmid alone (125 μg or 62.5 μg) or K3 plasmid with carrier PTAI-11/DOPE/DC (denoted as PTAI) or Lipofectin (L) in different w/w ratios indicated in brackets; pCI-125 – group vaccinated with empty pCI vector with Lipofectin. a ELISA results for individual chickens; raw data (◊), median (—) and the 10th and 90th percentiles (□) are shown for each group; blood collected at indicated day post initial immunization, sera diluted 200-fold. (*) group showed significantly higher ELISA values than K3-62.5. (**) group showed significantly higher ELISA values than K3-62.5 and K3-62.5/L (p < 0.05). b HI titers in sera of individual chickens on day 28 post immunization. Geometric means for each group are indicated at the top of the graph. (*) The K3-62.5/PTAI (0.8) group showed a significantly higher HI titer than K3-62.5 (p < 0.05)