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Fig. 1 | Virology Journal

Fig. 1

From: The effect of bovine BST2A1 on the release and cell-to-cell transmission of retroviruses

Fig. 1

bBST2A1 inhibits release of retroviruses. (a) HA-bBST2A1 and HA-hBST2 expressing plasmids were transfected into HEK293T cells. Expression of BST2 orthologues were analyzed by western blotting 48 h post-transfection. (b-c) HIV-1 molecular clone NL4–3.E-U- and HIV-1 Env expressing plasmids were transfected into HEK293T cells together with vector control, HA-bBST2A1 or HA-hBST2 expressing plasmid. 48 h post-transfection, cells and culture supernatants were subjected to western blotting using HIV-1 p24 antibody (b). Supernatants were also used to infect TZM-bl indicator cells, followed by luciferase assays (c). (d-e) HEK293T cells were transfected with pcPFV (d) or pcBFV (e), together with BST2 plasmids or vector control. 48 h post-transfection, cells were harvested for western blotting, and supernatants were collected and used to infect PFVL (d) or BFVL (e) indicator cells. Data of three independent experiments are summarized in the bar graph. Data are represented as mean ± SEM

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