Fig. 4
From: Membrane-anchored CCL20 augments HIV Env-specific mucosal immune responses
Effect of CCL20 on the level of mucosal IgA-ASCs. Distribution of Env-specific IgA ASCs at the mucosal level in BALB/c mice immunized with various HIV VLPs by different routes i.m., i.n., IV is shown in panel a. Lymphocytes were prepared from lamina propria of small intestine. Effect of blocking CCL20 on intestinal homing of Env-specific IgA ASCs was shown in panel b. BALB/c mice were immunized with HIV VLPs in different routes and also injected i.p. with PBS or anti-CCL20 mAb. After 6 days, lymphocytes were prepared from lamina propria of small intestine. IgA ASCs were enumerated by ELISPOT. The results of ELISPOT images were from cVLPs immunized groups. Data represent mean ± SD and statistically significant differences are represented