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Table 1 primer sequences

From: Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

primer name sequence restriction enzyme
UL24/N-F 5′-AGGAGGACCTGCATATGATGGCTTCTAAGGTTCAAAAGAAGAGA-3’ Nde I
UL24/N-R 5′-GGATCCCCGGGAATTCTGGTATTCAGACAAACCAG-3’ EcoR I
UL24/C-F 5′-ATCGCTGGTTTGTCTGAATACCACATACCTACCAAAGGTAAGCGCCGG-3’ ——
UL24/C-R 5′-GGATCCCCGGGAATTCCTAGTGTTTAGTTGGTCTGA EcoR I
pCMV-myc-UL24 F 5′-ATGGAGGCCCGAATTCGGATGGCATCGAAGGTACAGA-3’ EcoR I
pCMV-myc-UL24 R 5′-GCCGCGGTACCTCGAGACTAGTGTTTAGTTGGTCTGAA-3’ Xho I
pCMV-Flag-UL54 F 5′-CATATGATGGCCTGCAGTGCTAAA-3’ Nde I
pCMV-Flag-UL54 R 5′-GGATCCCAAACATTTCATTACAATAAAA-3’ BamH I
pEGFP-N1-UL24-F1 5′-AAGCTTCGAATTCTGATGGCATCGAAGGTACAGA-3’ EcoR I
pEGFP-N1-UL24-R2 5′-CGACCGGTGGATCCCGGGCGTGTTTAGTTGGTCTGAATA-3’ Sma I
pDsRed-N1-UL54-F1 5′- TCTCAAGCTTAAGCTATGGCCTGCAGTGCTAAAC-3’ Hind III
pDsRed-N1-UL54 R2 5′- GGCGACCGGTGAGCTCGTAAACATTTCATTACAATA-3’ BamH I
  1. The restriction enzyme sites were bold