Table 1 primer sequences
From: Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein
primer name | sequence | restriction enzyme |
|---|---|---|
UL24/N-F | 5′-AGGAGGACCTGCATATGATGGCTTCTAAGGTTCAAAAGAAGAGA-3’ | Nde I |
UL24/N-R | 5′-GGATCCCCGGGAATTCTGGTATTCAGACAAACCAG-3’ | EcoR I |
UL24/C-F | 5′-ATCGCTGGTTTGTCTGAATACCACATACCTACCAAAGGTAAGCGCCGG-3’ | —— |
UL24/C-R | 5′-GGATCCCCGGGAATTCCTAGTGTTTAGTTGGTCTGA | EcoR I |
pCMV-myc-UL24 F | 5′-ATGGAGGCCCGAATTCGGATGGCATCGAAGGTACAGA-3’ | EcoR I |
pCMV-myc-UL24 R | 5′-GCCGCGGTACCTCGAGACTAGTGTTTAGTTGGTCTGAA-3’ | Xho I |
pCMV-Flag-UL54 F | 5′-CATATGATGGCCTGCAGTGCTAAA-3’ | Nde I |
pCMV-Flag-UL54 R | 5′-GGATCCCAAACATTTCATTACAATAAAA-3’ | BamH I |
pEGFP-N1-UL24-F1 | 5′-AAGCTTCGAATTCTGATGGCATCGAAGGTACAGA-3’ | EcoR I |
pEGFP-N1-UL24-R2 | 5′-CGACCGGTGGATCCCGGGCGTGTTTAGTTGGTCTGAATA-3’ | Sma I |
pDsRed-N1-UL54-F1 | 5′- TCTCAAGCTTAAGCTATGGCCTGCAGTGCTAAAC-3’ | Hind III |
pDsRed-N1-UL54 R2 | 5′- GGCGACCGGTGAGCTCGTAAACATTTCATTACAATA-3’ | BamH I |