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Fig. 3 | Virology Journal

Fig. 3

From: Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

Fig. 3

Co-immunoprecipitation of UL24 and UL54. HEK293T cells were co-transfected with eukaryotic plasmids (pCMV-myc-UL24 and pCMV-Flag-UL54, pCMV-myc-UL24 and pCMV-Flag respectively). At 48 h post-transfection, rabbit anti-UL24 pAb were incubated with the extracts from the cells co-transfected with the plasmids pCMV-myc-UL24 and pCMV-Flag-UL54; Abs against myc (mouse against myc) were incubated with extracts from the cells co-transfected with pCMV-myc-UL24 and pCMV-Flag-UL54, pCMV-myc-UL24 and pCMV-Flag-N respectively. Immunoprecipitated complexes were analyzed by western blotting with mouse anti-Flag pAb. The above transfected 293 T cell extracts were analyzed by western blotting with mouse anti-Flag pAb to detect the expression of Flag-UL54

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