Fig. 1
From: Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein
Bait plasmid construction. a, the nucleotide sequence of UL24/C (721 ~ 1230 bp, lane 3) and codon optimized UL24/N (1 ~ 720 bp, lane 2) was firstly amplified by PCR. Then, the full length codon optimized UL24 gene (1 ~ 1230 bp, lane 4) was amplified with the DNA fragments UL24/C and UL24/N as template by overlap PCR. M4500: DNA Marker 200 ~ 4500 bp. DL2000: DNA Marker 100 ~ 2000 bp. b, “bait” proteins (N and FL) were expressed in Saccharomyces cerevisiae Y2HGold strain according to be detected by western blotting. Primary antibody (mouse-anti myc mAb) is diluted 1000 folds, and HRP-goat anti-mouse antibody is diluted 80,000 folds. Immunoreactive proteins are detected using the ECL kit (enhanced chemiluminescence system, Bio-Rad)