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Fig. 1 | Virology Journal

Fig. 1

From: Rapid evolution of the PB1-F2 virulence protein expressed by human seasonal H3N2 influenza viruses reduces inflammatory responses to infection

Fig. 1

Full length PB1-F2 protein matching the 1968 pandemic isolate contributes to enhancement of inflammatory response to infection. BALB/cJ mice (n = 10, female, 8-10wk) were infected with 100 PFU reverse engineered A/Udorn/307/1972 virus containing the PB1 gene of the 1968, 1972, 1999 and 2010 isolates, or matched viruses genetically altered to produce 34 AA, or in the case of the 2010 strain a 90 AA, PB1-F2 protein. a) Viral load (PFU/mL) of homogenised lung tissue taken at 72 hpi was not significantly different between groups (p > 0.05, one-way ANOVA). BAL samples were taken at 72 hpi and examined for b leukocyte, c alveolar macrophage and d neutrophil content. * p < 0.05, *** p < 0.001 One-way ANOVA, Sidak’s multiple comparison test

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