Respiratory syncytial virus (RSV) entry is inhibited by serine protease inhibitor AEBSF when present during an early stage of infection

Table 1 Overview of protease inhibitors used

Protease inhibitor	Characteristics	Cell permeable	Typical Working concentrations	Used working concentrations	References
E-64	Cysteine protease inhibitor that also inhibits trypsin	YES	1 – 10 μM	1 – 30 μM	Product data; [40, 41]
Pepstatin A	Aspartic protease inhibitor such as renin, chymosin and pepsin	INEFFICIENT	1 μM	1 – 30 μM	Product data; [40, 42]
AEBSF	Broad spectrum serine protease inhibitor	YES	0.1–1.0 mM	0.1–0.5 mM	Product data; [40, 43, 44]
Aprotinin	Serine protease inhibitor that does not inhibit thrombin	NO	0.3 μM	0.8 – 8 μM	Product data; [40, 45]
PMSF	Broad spectrum serine protease inhibitor	YES	0.1–1.0 mM	0.1 – 3 mM	Product data; [46, 47]
TPCK	Inhibits chymotrypsin-like serine proteases	YES	10 – 100 μM	3 – 30 μM	Product data; [48, 49]

ISSN: 1743-422X