Fig. 5

Sensitivity of clinical RSV isolates to AEBSF treatment. HEp-2 cells were infected with RSV A2, or clinical isolates RSV A1998 3–2 or RSV A2000 3–4. Cells were infected for 2 h at 37 °C in the presence of 0 mM, 0.1 mM and 0.3 mM AEBSF and further incubated for 16 h with the same concentration of inhibitor. Afterwards, Cells were fixed, permeabilized, incubated with a pAb Goat Anti-RSV antibody and stained with AF488 Donkey anti-Goat (IgG). Nuclei were visualized with DAPI and analyzed with fluorescence microscopy. The percentage of RSV positive cells was calculated as the number of positive cells relative to the total number of nuclei. Results are expressed as means (±SEM) (n = 3), significant differences compared to the untreated infection control (0 mM) are indicated by ***p < 0.001