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Fig. 3 | Virology Journal

Fig. 3

From: Respiratory syncytial virus (RSV) entry is inhibited by serine protease inhibitor AEBSF when present during an early stage of infection

Fig. 3

AEBSF treatment during RSV infection in different cell lines. HEp-2 cells, A549 cells and BEAS-2B cells were pretreated with AEBSF for 1 h, infected with RSV A2 in the presence of AEBSF and incubated with inhibitor for 16 h. Afterwards cells were fixed, permeabilized, incubated with a pAb goat anti-RSV antibody and stained with AF488 donkey anti-goat (IgG) (Green). Nuclei were visualized with DAPI (Blue) and analyzed with fluorescence microscopy. Infected cells were counted relative to the total number of nuclei. a Representative images of the treated and infected cells of each cell line. b Percentage of RSV positive cells after treatment with 0 mM, 0.1 mM or 0.3 mM AEBSF respectively. Data for each cell line represents means (±SEM) of 3 independent repeats, significant differences compared to the untreated control (0 mM) are indicated by ***p < 0.001

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