Fig. 1

Single infection cycle. HEp-2 cells were infected with RSV A2 at 4 °C for 2 h before shifting them to 37 °C. Cells were fixed at different timepoints, permeabilized and stained with a primary antibody pAb goat anti-RSV antibody and secondary antibody Alexa fluor 488 (AF488) donkey anti-goat (IgG) (green). Nuclei were visualized with DAPI (blue). a Infection kinetics of RSV A2. Infected cells were counted at each indicated time point with fluorescence microscopy relative to the total number of nuclei plotted as percentage of RSV positive cells. b Fluorescent images (objective 20× magnification) of important timepoints during the infection kinetics