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Fig. 3 | Virology Journal

Fig. 3

From: Small-size recombinant adenoviral hexon protein fragments for the production of virus-type specific antibodies

Fig. 3

HAdV-C5 HVRs 1-6 hexon fragment efficiently blocks the neutralizing activity of an anti HAdV-C5 antibody. a, b Titration of the HAdV-C5 neutralizing serum. Five-fold serial dilutions of the neutralizing serum were pre-incubated with either HAdV-C5-CMVLuc or HAdV-B3-CMVLuc for 30 min before adding to human A549 cells. Luciferase activity was determined as relative light unit signal (RLU) in lysed cells 24 h post infection. c-f Blocking of HAdV-C5 neutralizing activity by the HAdV-C5 HVRs 1-6 hexon fragment. Two neutralizing antibody dilutions, 1:250 and 1:1250, which gave rise to <0.075% and ~5% residual HAdV-C5 activity, respectively, were used to repeat the neutralization assays in the presence of increasing amounts of hexon fragment. Blocking of neutralization could be seen in the homologous system using HAdV-C5-CMVLuc, but not in the heterologous system for HAdV-B3-CMVLuc-mediated expression

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