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Fig. 2 | Virology Journal

Fig. 2

From: Small-size recombinant adenoviral hexon protein fragments for the production of virus-type specific antibodies

Fig. 2

Purification of recombinant adenoviral hexon fragments. a Schematic representation of the purification process. Transformed E.coli BL21-CodonPlus(DE3)-RIPL cells were lysed to release cellular contents. The recombinant GST-fusion proteins were pulled down using Glutathione-Sepharose beads. After elution, the GST tag was cleaved and the hexon fragments were further purified using Mono Q and Q5 ion exchange chromatography. The resulting protein fractions were concentrated. b-e SDS-PAGE analyses of 1, 2 and 4 μl of purified HAdV-B3, HAdV-C5, MAdV-1 and MAdV-2 hexon fragments. Arrows indicate the bands of the purified proteins

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