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Fig. 5 | Virology Journal

Fig. 5

From: The impact of p53 on the early stage replication of retrovirus

Fig. 5

Analysis of SAMHD1 and p21Cip1 gene expression in HCTp53+/+ and HCT116 p53−/− cells. 2.5 × 105 cells of non-cycling HCT 116 p53+/+ and HCT116 p53−/− cells cultured in DMEM with 0.5% FBS (Un-infected), and were infected with 0.5 ml 2.5 × 107 copies/ml retrovirus for 4 h, 8 h and 16 h. RNA were extracted from cells harvested, and then analyzed by p21Cip1 TaqMan real time PCR (a), SAMHD1 TaqMan real time PCR (b). The copy numbers of mRNA were normalized to reference gene GAPDH. p53, p21Cip1, SAMHD1, phospho-SAMHD1 (Thr592) and β-actin proteins were analyzed by Western blot (c) . p-value of Student’s t-test is indicated. p value < 0.01 is indicated by **

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