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Fig. 2 | Virology Journal

Fig. 2

From: The impact of p53 on the early stage replication of retrovirus

Fig. 2

Quantification of Late RT and 2-LTR Cycle Viral DNA in Retrovirus Infected Non-Cycling HCT 116 p53+/+ and HCT116 p53−/− Cells. 2.5 × 105 cells of non-cycling HCT 116 p53+/+ and HCT116 p53−/− cells were infected with 0.5 ml 5 × 107 copies/ml retrovirus. The DNA were extracted in infected cells at 4 h, 8 h, 16 h and 24 h post infection. A heat inactivated virus (Inact-V) was used as negative control. The amount of viral late RT (a) and 2-LTR (b) in extracted DNA were quantified by SRBR Green real time PCR. The ratios of the amount between 2-LTR and RT (2LTR/RT) in infected cells were also calculated (c). The results represented a triplicate experiment. In Student’s t-test, p value < 0.05 is indicated by *; p value < 0.01 is indicated by **

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