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Fig. 1 | Virology Journal

Fig. 1

From: The impact of p53 on the early stage replication of retrovirus

Fig. 1

Analysis of Retrovirus Infection in Cycling and Non-Cycling HCT p53+/+ and HCT116 p53−/− Cells. a. Flow cytometer analysis of cell cycle of cycling and non-cycling HCT p53+/+ and HCT116 p53−/− cells. Non-cycling cells were cultured in DMEM with 0.5% FBS for 48 h. Cells are stained with propidium iodide (PI). b. Flow cytometer analysis of retrovirus infection in cycling HCT p53+/+ and HCT116 p53−/− cells. Uninfected cells were shown as the 1st peak, and the infected GFP+ cells were shown as the 2nd peak. 0.5 ml of 5 × 107 (I), 2.5 × 107 (II) and 1.25 × 107 (III) copies/ml of VSV-G pseudotyped retrovirus were used to infect 2.5 × 105 cells in each well in a 24 well plate. c. The percentage of GFP+ positive cells were quantified by a flow cytometer after infection of non-cycling HCT p53+/+ and HCT116 p53−/− by 0.5 ml of 5 × 107 (I) copies/ml of VSV-G pseudotyped retrovirus, and the cell numbers (d) and viabilities (e) of the uninfected and infected cells were determined. The results represented a triplicate experiment. In Student’s t-test, p value < 0.05 is indicated by *

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