Co-infection of two reoviruses increases both viruses accumulation in rice by up-regulating of viroplasm components and movement proteins bilaterally and RNA silencing suppressor unilaterally

Li, Shu; Zhang, Tong; Zhu, Yingzhi; Zhou, Guohui

doi:10.1186/s12985-017-0819-0

Virology Journal

Table 1 Reverse-transcription quantitative polymerase chain reaction primers used for quantification of southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV)

From: Co-infection of two reoviruses increases both viruses accumulation in rice by up-regulating of viroplasm components and movement proteins bilaterally and RNA silencing suppressor unilaterally

Purpose	Gene name	Target gene (GenBank accession no.)	Primer for RT-qPCR(5′-3′)	Size of expected amplicon (bp)
RRSV	RRSV P1	HM125559	AGGATCTCTTCTCAATGCAAGC	70
	RRSV P1	HM125559	AACTTCCACCTGGCAACGTC	70
	RRSV P2	HM125560	GGCGTTCGATCTCGTGTTTAA	69
	RRSV P2	HM125560	GCGGGAAAATCATTGGCGTG	69
	RRSV P3	AF020336	TCAAAGCTAGTGATGAGCGTCTGT	70
	RRSV P3	AF020336	CTGGCATAAAAACGAAGAGTCTGA	70
	RRSV P4A	HM125562	GGCGTCAGTGTGTAGCAGCAT	66
	RRSV P4A	HM125562	ATTGGGTAGAGTTGTTGTTTTGGAT	66
	RRSV P4B	HM125562	CGCCATCCACAAAGCTATCA	63
	RRSV P4B	HM125562	AACACTCTCGTAGCCTGCCAAT	63
	RRSV P5	HM125562	CGCCATCCACAAAGCTATCA	59
	RRSV P5	HM125562	AACACTCTCGTAGCCTGCCAAT	59
	RRSV S5	HM125543	GGCGGCATCGGGTTGT	59
	RRSV S5	HM125543	CACCTCTATCAAACGCAGTAACCA	59
	RRSV S6	HM125554	GCTTTCGCGGTGCTCAA	59
	RRSV S6	HM125554	CTCCCAATTACGCACCGAAT	59
	RRSV P7	HM125555	TGACGATTACGCCGAGACAAG	62
	RRSV P7	HM125555	CGGACGACTGGCCAATG	62
	RRSV P8	HM125546	GGCTGAGCGTGCGGTTA	66
	RRSV P8	HM125546	TCAGCCTTGATATCGTTGTAGCA	66
	RRSV P9	L79969	GGTGTGGCTCTACAACAAATGG	67
	RRSV P9	L79969	ACGCCTCTTTCTCTGCTCCTCTA	67
	RRSV P10	HM125568	CCGAGCCGCCATCATAGTA	63
	RRSV P10	HM125568	ACCTCAAAAACTCCAGACAGCATA	63
SRBSDV	SRBSDV P1	JQ692572	TGTGATTTGAACTCAGAGACAGCTTTA	80
	SRBSDV P1	JQ692572	TGACGTTGATAAGTTGATTGTTGAGA	80
	SRBSDV P2	HM585278	GCGAACGGCTTTCTGCTTT	71
	SRBSDV P2	HM585278	GAATGTGCGAGTGATTTCATGAA	71
	SRBSDVP3	HM585277	TCCCGGTGTTCGAGAAGTTC	64
	SRBSDVP3	HM585277	CAGCATTTTCCATCCAACCAT	64
	SRBSDV P4	HM585276	TCAACGCTCGACAACCAAAA	72
	SRBSDV P4	HM585276	TTATCGTGATATCTGCTACGAATACCA	72
	SRBSDV P5-1	HM585275	ATCAGATGATAACCTCAATCGTACCA	94
	SRBSDV P5-1		TGAGCCAGTGAAGGTAATCATCATTA	94
	SRBSDV P5-2		CGCTTAACAATTGTCGTGATATGAA	75
	SRBSDV P5-2		GCGTGCTTCCGAAAAGTAAAGTT	75
	SRBSDV P6	HM585274	AAGCAACAAACATCAACGTCAAA	69
	SRBSDV P6	HM585274	AACTACGTCGGCCCATGAAC	69
	SRBSDV P7-1	HM58527	CCTAATGAAAACCCATCTACCTGTAAC	70
	SRBSDV P7-1		CACGAATAAACATCAGCGACAAA	70
	SRBSDV P7-2		CAAATATTTAGTAGAAGCAGAACGCAAGT	61
	SRBSDV P7-2		ACATCTTTTTCGTCTTTCTCATACTCTTAA	61
	SRBSDV P8	HM585272	GATTGTCTCCTTTGGATGATGTTG	72
	SRBSDV P8	HM585272	CCTGTCCTTCTTGAAATACACGTAAC	72
	SRBSDV P9-1	HM585271	TGAAAGCGAATCCTCAACTAAAGA	68
	SRBSDV P9-1		TTGAATCAGTGGAGGTGGGTTT	68
	SRBSDV P9-2		CGAGATGAGCGATTCTGGTTT	70
	SRBSDV P9-2		TTCGTGAATGATGTTGTTAACCTTTT	70
	SRBSDV P10	EU784840	TCATCATTAGCGCGACTAGTTCA	64
	SRBSDV P10	EU784840	CGTCACTCGGCGTCGATAA	64
Reference	U6	S83742	CGATAAAATTGGAACGATACAGA	72
Reference	U6	S83742	ATTTGGACCATTTCTCGATTTGT	72

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