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Fig. 1 | Virology Journal

Fig. 1

From: A novel method for transmitting southern rice black-streaked dwarf virus to rice without insect vector

Fig. 1

The SRBSDV transmission process using the bud-cutting method. a The germinated rice seed. (a) The germ, (b) The radicle, (c) The cotyledon, (d) The position (a cyclic structure) for cutting bud at a 45° angle approximately 0.5 cm from the base of the bud. b The bud-cutting rice seed. c The rice stems which were had obvious tumor-like protrusion symptoms. d The rice stems were frozen in liquid nitrogen and ground to obtain a fine powder. e The treated seedlings were soaked in the crude SRBSDV extract for 30 min to inoculate the seedlings with SRBSDV. f The seedlings were incubated in the dark at 28–30 °C for 3 days

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