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Fig. 3 | Virology Journal

Fig. 3

From: An equine herpesvirus type 1 (EHV-1) vector expressing Rift Valley fever virus (RVFV) Gn and Gc induces neutralizing antibodies in sheep

Fig. 3

Expression of Gn-Gc protein after infection with parental rH and rH_Gn-Gc virus. a Immunofluorescence staining of RK13 cells infected with either parental rH or rH_Gn-Gc virus. Plaques were stained with anti-RVFV(IN) (i and ii) or anti-RVFV(CT) (iii and iv) pAb, that was reactive with RVFV Gn or Gc, respectively, followed by Alexa Flour488-conjugated goat anti-rabbit IgG. Plaques in infected cells with rH_Gn-Gc visualized by fluorescence microscopy (i and iii), whereas those induced by rH virus were not (ii and iv). b Cell lysates either mock infected, infected by rH or rH_Gn-Gc were separated by 10% SDS-PAGE and analysed by Western blot. Expression of Gn and Gc was detected using pAb-RVFV(IN) (left panel) and -RVFV(CT) (right panel), respectively. Expression of β-actin was determined as a loading control. The PageRulerTM Prestained protein ladder (Thermo Scientific) was used for determination of protein sizes

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