Real-time reverse transcription PCR-based sequencing-independent pathotyping of Eurasian avian influenza A viruses of subtype H7

Table 3 Analytical performance characteristics of real time RT-PCR (RT-qPCR) assays for sequencing-independent pathotyping of Eurasian reference H7 viruses

Reference virus	Accession number of HA	Sub- and pathotype	RT-qPCR method
Reference virus	Accession number of HA	Sub- and pathotype	LPAI H7	HPAI H7 ‘Emsland’	HPAI H7 ‘Italy’
A/mute swan/Germany/R901/2006	EPI359695	LP H7N7	Pos	Neg	Neg
A/Anhui/1/2013	AHZ60096	LP H7N9	Pos	Neg	Neg
A/chicken/Germany/AR1385/2015	SA	HP H7N7	Neg	Pos	Neg
A/broiler/Italy/445/1999	AJ580353	HP H7N1	Neg	Neg	Pos
A/turkey/Germany/R2025/2008	SA	LP H5N3	Neg	Neg	Neg
A/turkey/Germany/AR2485–86/2014	EPI552746	HP H5N8	Neg	Neg	Neg
A/chicken/Egypt/AR753–14/2013	EPI557457	HP H9N2	Neg	Neg	Neg
A/chicken/Sudan/AR251–15/2014	KX272465	IBV	Neg	Neg	Neg
A/chicken/Egypt/AR254–15/2014	SA	NDV	Neg	Neg	Neg

LP low pathogenic, HP high pathogenic, Neg negative, Pos positive, SA sequence available from the authors, also represented in the alignment in Additional file 1: Figure S1, IBV Infectious bronchitis virus, NDV Newcastle disease virus

ISSN: 1743-422X