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Fig. 5 | Virology Journal

Fig. 5

From: Unexpected complexity in the interference activity of a cloned influenza defective interfering RNA

Fig. 5

Analysis of influenza segment 1-directed RNA synthesis by primer extension in the presence of influenza 1/244 DI RNA, segment1 RNA, or segment 6 RNA. 293T cells were transfected with plasmids as described in Fig. 3. Primer extension analysis of viral RNA directed by segment 1-GFP in the absence or presence of increasing amounts of plasmid encoding 1/244 DI RNA (panel a), genome segment 1 vRNA (c) or genome segment 6 (e). 5S rRNA detected from the same RNA preparations were used as an internal control. The primer extension products are identified on the left of each panel. Quantitation of viral RNA from three independent experiments by phosphorimaging analysis is shown in panels (b), (d), and (f). The values of band intensities were normalised against the relevant 5S rRNA and are expressed as a percentage of the maximum value for each RNA analysed. Basal levels of vRNA generated from the target plasmid were subtracted from the total as decribed in the text. The error bars represent the standard error of the mean of at least 3 replicates. vRNA (■), mRNA (▲) and cRNA ()

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