Comparison of serological assays to titrate Hantaan and Seoul hantavirus-specific antibodies

Li, Weihong; Cao, Shouchun; Zhang, Quanfu; Li, Jiandong; Zhang, Shuo; Wu, Wei; Qu, Jing; Li, Chuan; Liang, Mifang; Li, Dexin

doi:10.1186/s12985-017-0799-0

Virology Journal

Table 1 PRNT, MNT, PPNT, IFA-virus and IFA-GP titres of convalescent sera from patients of HFRS

From: Comparison of serological assays to titrate Hantaan and Seoul hantavirus-specific antibodies

Sero-type^a	Patient ID	Diagnosis of HFRS^b				Reciprocal PRNT titre^c				Reciprocal MNT titre^c				Reciprocal PPNT titre^c						Reciprocal IFA titre^c
		Acute serum			Convalescent serum	IC50		IC80		IC50		IC80		IC50			IC80			IFA-virus		IFA-GP
		IgM	IgG	RNA	IgG	SEOV	HTNV	SEOV	HTNV	SEOV	HTNV	SEOV	HTNV	SEOV	HTNV	VSV	SEOV	HTNV	VSV	SEOV	HTNV	SEOV	HTNV
HTN	SD11	+	+	NT	+	20	80	<20	20	20	80	<20	20	80	320	<20	20	80	<20	80	320	20	80
	JX46	+	+	−	+	<20	80	<20	20	<20	80	<20	80	80	320	<20	20	80	<20	80	80	80	80
	SD15	+	+	NT	+	<20	80	<20	20	<20	80	<20	80	80	320	<20	20	80	<20	80	80	20	80
	LN264	+	−	NT	+	<20	80	<20	20	<20	80	<20	20	20	320	<20	20	80	<20	80	80	<20	20
	JX11	+	+	−	+	20	80	<20	80	<20	80	<20	80	20	320	20	<20	80	<20	80	80	20	80
	JX16	+	−	−	+	20	320	<20	80	20	320	<20	80	80	1280	<20	20	320	<20	80	80	20	80
	JX53	+	+	−	+	20	320	<20	80	20	320	20	80	80	320	<20	20	80	<20	20	20	20	80
	JX39	+	+	−	+	20	320	<20	80	<20	320	<20	80	20	1280	<20	20	320	<20	80	80	20	80
	JX36	+	+	−	+	<20	320	<20	80	<20	320	<20	80	20	320	20	20	80	<20	80	320	20	80
	SD7	+	+	−	+	<20	320	<20	80	<20	320	<20	80	320	320	<20	80	80	<20	<20	80	80	80
	SD14	+	+	−	+	<20	320	<20	80	<20	320	<20	80	80	1280	<20	20	320	<20	<20	80	20	80
	SD39	+	+	−	+	<20	320	<20	80	<20	80	<20	80	80	320	<20	20	80	<20	80	80	20	80
	SD21	+	+	H+	+	20	320	20	80	<20	320	<20	80	320	1280	<20	80	320	<20	320	320	80	80
	SD31	+	+	−	+	20	320	<20	80	20	320	<20	80	320	5120	<20	80	320	<20	1280	1280	80	320
	SD38	+	+	−	+	<20	320	<20	80	<20	320	<20	80	80	1280	<20	20	320	<20	80	80	20	80
	JX12	+	+	−	+	<20	320	<20	80	<20	320	<20	80	80	1280	<20	20	320	<20	20	20	20	80
	JX60	+	+	NT	+	20	1280	<20	320	20	1280	<20	320	80	5120	<20	20	1280	<20	320	320	80	320
	JX35	+	+	−	+	<20	1280	<20	320	20	1280	<20	320	320	1280	<20	80	320	<20	80	80	20	80
	SD27	+	+	−	+	20	1280	20	320	20	5120	20	1280	80	5120	<20	20	1280	<20	80	320	80	320
	SD25	+	+	−	+	80	1280	20	320	80	1280	20	320	320	1280	<20	80	320	<20	320	320	80	320
	SD33	+	−	−	+	80	1280	20	320	20	1280	<20	320	320	1280	<20	80	320	<20	320	320	80	320
	SD36	+	+	−	+	<20	1280	<20	320	20	1280	<20	320	320	1280	<20	80	320	<20	80	80	80	80
	SD26	+	+	−	+	<20	1280	<20	320	<20	1280	<20	320	80	1280	<20	20	320	<20	20	80	20	80
	JX3	+	+	−	+	20	1280	<20	320	<20	1280	<20	320	80	1280	<20	20	320	<20	320	320	80	320
	SD29	+	+	H+	+	80	1280	20	320	20	1280	20	320	320	5120	20	80	1280	<20	80	320	20	80
	SD20	+	+	−	+	320	1280	80	320	320	1280	80	320	1280	5120	<20	320	1280	<20	80	80	320	320
	LN34	+	+	NT	+	1280	20,480	320	5120	1280	20,480	320	5120	5120	20,480	20	1280	5120	<20	320	320	5120	5120
SEO	LN216	+	+	NT	+	80	20	20	<20	320	20	80	<20	320	80	<20	80	20	<20	80	80	80	20
	LN28	+	−	NT	+	80	20	20	<20	80	20	20	20	80	<20	<20	20	<20	<20	320	80	80	<20
	LN266	+	+	NT	+	80	20	20	<20	320	<20	80	<20	320	80	<20	80	20	<20	80	80	80	20
	LN265	+	+	−	+	80	20	20	20	80	20	80	20	320	20	<20	80	<20	<20	80	80	80	<20
	LN263	+	+	−	+	80	20	20	20	320	<20	80	<20	320	80	<20	80	20	<20	80	80	80	<20
	LN262	+	+	S+	+	80	20	20	20	320	<20	80	<20	1280	<20	<20	320	<20	<20	320	80	320	20
	JX1	+	−	−	+	320	20	80	20	320	20	80	<20	1280	80	20	80	20	<20	320	320	80	20
	SD4	+	+	−	+	320	80	80	20	320	20	320	<20	1280	20	20	320	<20	<20	80	80	80	<20
	SD2	+	+	−	+	320	20	80	<20	320	<20	80	<20	320	<20	20	80	<20	<20	320	320	80	<20
	JX41	+	+	NT	+	320	<20	80	<20	1280	<20	320	<20	320	80	<20	80	20	<20	80	20	80	20
	SD30	+	+	−	+	320	<20	80	<20	320	<20	80	<20	1280	20	20	320	<20	<20	80	20	80	20
	SD1	+	+	S+	+	320	<20	80	<20	320	20	320	20	1280	20	<20	320	<20	<20	320	320	320	80
	LN27	+	+	NT	+	1280	20	320	<20	1280	<20	320	<20	1280	<20	<20	320	<20	<20	1280	80	320	20
	LN249	+	+	S+	+	1280	320	320	80	1280	80	1280	20	5120	80	<20	1280	20	<20	320	320	1280	320
	SD37	+	+	−	+	5120	320	5120	80	20,480	1280	5120	320	20,480	320	20	5120	80	<20	80	80	1280	320
Unknown	JX22	+	+	NT	+	20	20	<20	<20	<20	20	<20	20	80	80	<20	20	20	<20	80	80	20	20
Unknown	SD40	+	+	−	+	320	320	80	80	320	320	80	80	1280	1280	20	320	320	<20	80	80	80	80

^aSerotype of infection was determined to be HTN (Hantaan), SEO (Seoul) or Unknown by whether antibody level against the homotypic virus was ≥4-fold higher than that of the heterotypic virus
^bHFRS patients were confirmed by using recombinant nucleocapside proteins based IgM capture ELISA [37] or IgG ELISA [38] for Hantaan virus (HTNV) and Seoul virus (SEOV), and viral RNA was detected by real-time RT-PCR [39] to confirm genotype of infection. +, Positive result. −, Negative result. NT, Not tested. H+, HTNV RNA positive. S+, SEOV RNA positive
^cConvalescent sera from laboratory confirmed patients of hemorrhagic fever with renal syndrome (HFRS) were titrated against HTNV strain 84FLi and SEOV strain L99 by plaque reduction neutralization test (PRNT), microneutralization test (MNT), pseudoparticle neutralization test (PPNT), immunofluorescence assay based on virus-infected cells(IFA-virus) and immunofluorescence assay based on viral glycoproteins (IFA-GP). PRNT, MNT, PPNT and IFA titres were expressed as the reciprocal of the highest dilution of serum resulting in specific reduction of the number of virus plaques or absorbance value or luciferase activity or fluorescence by 50% (IC50) and 80% (IC80), respectively. IC50, 50% inhibition concentration. IC80, 80% inhibition concentration

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ISSN: 1743-422X

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