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Table 1 PRNT, MNT, PPNT, IFA-virus and IFA-GP titres of convalescent sera from patients of HFRS

From: Comparison of serological assays to titrate Hantaan and Seoul hantavirus-specific antibodies

Sero-typea Patient ID Diagnosis of HFRSb Reciprocal PRNT titrec Reciprocal MNT titrec Reciprocal PPNT titrec Reciprocal IFA titrec
Acute serum Convalescent serum IC50 IC80 IC50 IC80 IC50 IC80 IFA-virus IFA-GP
IgM IgG RNA IgG SEOV HTNV SEOV HTNV SEOV HTNV SEOV HTNV SEOV HTNV VSV SEOV HTNV VSV SEOV HTNV SEOV HTNV
HTN SD11 + + NT + 20 80 <20 20 20 80 <20 20 80 320 <20 20 80 <20 80 320 20 80
JX46 + + + <20 80 <20 20 <20 80 <20 80 80 320 <20 20 80 <20 80 80 80 80
SD15 + + NT + <20 80 <20 20 <20 80 <20 80 80 320 <20 20 80 <20 80 80 20 80
LN264 + NT + <20 80 <20 20 <20 80 <20 20 20 320 <20 20 80 <20 80 80 <20 20
JX11 + + + 20 80 <20 80 <20 80 <20 80 20 320 20 <20 80 <20 80 80 20 80
JX16 + + 20 320 <20 80 20 320 <20 80 80 1280 <20 20 320 <20 80 80 20 80
JX53 + + + 20 320 <20 80 20 320 20 80 80 320 <20 20 80 <20 20 20 20 80
JX39 + + + 20 320 <20 80 <20 320 <20 80 20 1280 <20 20 320 <20 80 80 20 80
JX36 + + + <20 320 <20 80 <20 320 <20 80 20 320 20 20 80 <20 80 320 20 80
SD7 + + + <20 320 <20 80 <20 320 <20 80 320 320 <20 80 80 <20 <20 80 80 80
SD14 + + + <20 320 <20 80 <20 320 <20 80 80 1280 <20 20 320 <20 <20 80 20 80
SD39 + + + <20 320 <20 80 <20 80 <20 80 80 320 <20 20 80 <20 80 80 20 80
SD21 + + H+ + 20 320 20 80 <20 320 <20 80 320 1280 <20 80 320 <20 320 320 80 80
SD31 + + + 20 320 <20 80 20 320 <20 80 320 5120 <20 80 320 <20 1280 1280 80 320
SD38 + + + <20 320 <20 80 <20 320 <20 80 80 1280 <20 20 320 <20 80 80 20 80
JX12 + + + <20 320 <20 80 <20 320 <20 80 80 1280 <20 20 320 <20 20 20 20 80
JX60 + + NT + 20 1280 <20 320 20 1280 <20 320 80 5120 <20 20 1280 <20 320 320 80 320
JX35 + + + <20 1280 <20 320 20 1280 <20 320 320 1280 <20 80 320 <20 80 80 20 80
SD27 + + + 20 1280 20 320 20 5120 20 1280 80 5120 <20 20 1280 <20 80 320 80 320
SD25 + + + 80 1280 20 320 80 1280 20 320 320 1280 <20 80 320 <20 320 320 80 320
SD33 + + 80 1280 20 320 20 1280 <20 320 320 1280 <20 80 320 <20 320 320 80 320
SD36 + + + <20 1280 <20 320 20 1280 <20 320 320 1280 <20 80 320 <20 80 80 80 80
SD26 + + + <20 1280 <20 320 <20 1280 <20 320 80 1280 <20 20 320 <20 20 80 20 80
JX3 + + + 20 1280 <20 320 <20 1280 <20 320 80 1280 <20 20 320 <20 320 320 80 320
SD29 + + H+ + 80 1280 20 320 20 1280 20 320 320 5120 20 80 1280 <20 80 320 20 80
SD20 + + + 320 1280 80 320 320 1280 80 320 1280 5120 <20 320 1280 <20 80 80 320 320
LN34 + + NT + 1280 20,480 320 5120 1280 20,480 320 5120 5120 20,480 20 1280 5120 <20 320 320 5120 5120
SEO LN216 + + NT + 80 20 20 <20 320 20 80 <20 320 80 <20 80 20 <20 80 80 80 20
LN28 + NT + 80 20 20 <20 80 20 20 20 80 <20 <20 20 <20 <20 320 80 80 <20
LN266 + + NT + 80 20 20 <20 320 <20 80 <20 320 80 <20 80 20 <20 80 80 80 20
LN265 + + + 80 20 20 20 80 20 80 20 320 20 <20 80 <20 <20 80 80 80 <20
LN263 + + + 80 20 20 20 320 <20 80 <20 320 80 <20 80 20 <20 80 80 80 <20
LN262 + + S+ + 80 20 20 20 320 <20 80 <20 1280 <20 <20 320 <20 <20 320 80 320 20
JX1 + + 320 20 80 20 320 20 80 <20 1280 80 20 80 20 <20 320 320 80 20
SD4 + + + 320 80 80 20 320 20 320 <20 1280 20 20 320 <20 <20 80 80 80 <20
SD2 + + + 320 20 80 <20 320 <20 80 <20 320 <20 20 80 <20 <20 320 320 80 <20
JX41 + + NT + 320 <20 80 <20 1280 <20 320 <20 320 80 <20 80 20 <20 80 20 80 20
SD30 + + + 320 <20 80 <20 320 <20 80 <20 1280 20 20 320 <20 <20 80 20 80 20
SD1 + + S+ + 320 <20 80 <20 320 20 320 20 1280 20 <20 320 <20 <20 320 320 320 80
LN27 + + NT + 1280 20 320 <20 1280 <20 320 <20 1280 <20 <20 320 <20 <20 1280 80 320 20
LN249 + + S+ + 1280 320 320 80 1280 80 1280 20 5120 80 <20 1280 20 <20 320 320 1280 320
SD37 + + + 5120 320 5120 80 20,480 1280 5120 320 20,480 320 20 5120 80 <20 80 80 1280 320
Unknown JX22 + + NT + 20 20 <20 <20 <20 20 <20 20 80 80 <20 20 20 <20 80 80 20 20
SD40 + + + 320 320 80 80 320 320 80 80 1280 1280 20 320 320 <20 80 80 80 80
  1. aSerotype of infection was determined to be HTN (Hantaan), SEO (Seoul) or Unknown by whether antibody level against the homotypic virus was ≥4-fold higher than that of the heterotypic virus
  2. bHFRS patients were confirmed by using recombinant nucleocapside proteins based IgM capture ELISA [37] or IgG ELISA [38] for Hantaan virus (HTNV) and Seoul virus (SEOV), and viral RNA was detected by real-time RT-PCR [39] to confirm genotype of infection. +, Positive result. −, Negative result. NT, Not tested. H+, HTNV RNA positive. S+, SEOV RNA positive
  3. cConvalescent sera from laboratory confirmed patients of hemorrhagic fever with renal syndrome (HFRS) were titrated against HTNV strain 84FLi and SEOV strain L99 by plaque reduction neutralization test (PRNT), microneutralization test (MNT), pseudoparticle neutralization test (PPNT), immunofluorescence assay based on virus-infected cells(IFA-virus) and immunofluorescence assay based on viral glycoproteins (IFA-GP). PRNT, MNT, PPNT and IFA titres were expressed as the reciprocal of the highest dilution of serum resulting in specific reduction of the number of virus plaques or absorbance value or luciferase activity or fluorescence by 50% (IC50) and 80% (IC80), respectively. IC50, 50% inhibition concentration. IC80, 80% inhibition concentration