Semi-quantitative Influenza A population averages from a multiplex respiratory viral panel (RVP): potential for reflecting target sequence changes affecting the assay

Table 2 Ct adjusted nAMPs and GenMark RVP Matrix Gene Sequence Mismatches

Influenza Strain	Matrix Gene nAMPs^a	Matrix Gene Mismatches
Influenza Strain	Matrix Gene nAMPs^a	Forward	Reverse	Capture	Signal
A/NY	53.5 ± 32^b	1^c	0	3^d	0
A/Texas	56.6 ± 11.4^b	1	0	3	0
A/Switzerland	46 ± 16.9^b	1	0	3	0
H3N2 Synthetic Capture Probe ssDNA^e	73 ± 16	N/A^f	N/A	3	N/A
H1N1	143.6 ± 57.2	1	0	2	0
H1N1 Synthetic Capture Probe ssDNA	151 ± 17	N/A	N/A	2	N/A

^aMean ± SD of 4 replicates. All 4 strains were diluted to the same copy number based on Ct using a TaqMan assay (see Methods)
^bA/NY (p = 0.033), A/Texas (p = 0.025) and A/Switzerland (p = 0.017) vs H1N1 t test. https://www.usablestats.com/calcs/2samplet
^cThe mismatches were the same in all H3N2 strains, but different from the H1N1
^dAll 3 mismatches were the same for the H3N2 strains, but differed from the 2 mismatches in the H1N1 strain
^essDNA = single stranded DNA
^fN/A = not applicable

ISSN: 1743-422X