Skip to main content

Advertisement

Table 2 Ct adjusted nAMPs and GenMark RVP Matrix Gene Sequence Mismatches

From: Semi-quantitative Influenza A population averages from a multiplex respiratory viral panel (RVP): potential for reflecting target sequence changes affecting the assay

Influenza Strain Matrix Gene nAMPsa Matrix Gene Mismatches
Forward Reverse Capture Signal
 A/NY 53.5 ± 32b 1c 0 3d 0
 A/Texas 56.6 ± 11.4b 1 0 3 0
 A/Switzerland 46 ± 16.9b 1 0 3 0
 H3N2 Synthetic Capture Probe ssDNAe 73 ± 16 N/Af N/A 3 N/A
 H1N1 143.6 ± 57.2 1 0 2 0
 H1N1 Synthetic Capture Probe ssDNA 151 ± 17 N/A N/A 2 N/A
  1. aMean ± SD of 4 replicates. All 4 strains were diluted to the same copy number based on Ct using a TaqMan assay (see Methods)
  2. bA/NY (p = 0.033), A/Texas (p = 0.025) and A/Switzerland (p = 0.017) vs H1N1 t test. https://www.usablestats.com/calcs/2samplet
  3. cThe mismatches were the same in all H3N2 strains, but different from the H1N1
  4. dAll 3 mismatches were the same for the H3N2 strains, but differed from the 2 mismatches in the H1N1 strain
  5. essDNA = single stranded DNA
  6. fN/A = not applicable