Table 2 Primers for amplifying the complete genome of ENTV-2
From: Full-length genome sequence analysis of enzootic nasal tumor virus isolated from goats in China
Primer | Primer sequence(5′-3′) | Location | Product length |
|---|---|---|---|
1F | ACAAGGCATCAGCCATTTTGGTCTGATCCTCTCAACCCCA | 1-40 | 627 bp |
1R | AGGAGGAGGAGCATCATAACCAGGCTCTGGGTCAGGAATA | 627-588 | |
2F | GTTTTCCTCGCCACTACTCTTG | 151-172 | 2249 bp |
2R | TACCCAATAAGCGTCGGATGAT | 2399-2378 | |
3F | CACTCCTAATTTGTGCCCACG | 1848-1869 | 1087 bp |
3R | GGCCACTGATCGACCCATAC | 2934-2915 | |
4F | GAAGAGGTTTGGGGTGTTTTCCCTAGGGACCTCTGATTCTCCTGTGAC | 2830-2878 | 2258 bp |
4R | GTTTAAGACGTTGATGAGCTCGTTCTACAATCCCTTGTCCCTGTGGGT | 5087-5040 | |
5F | AGAACGAGCTCATCAACGTCTTAAACATCAACT | 5062-5094 | 2379 bp |
5R | CTTGTTGTTTTATTGTGTCATAGTATATAT | 7440-7411 |