Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication

Table 1 Primers used in this study

Primers^a	Sequence (5^′-3′)^b	Use
1F	AGCTTTGTTTAAACCCATGTACCCATACGATGTTC	pWPXL-nsp9 construction
1R	CGACGCGTAACTCATGATTGGACCTGAG	pWPXL-nsp9 construction
2F	ATGAGGGGGGACAGAGGCCGTG	ILF2 gene amplification
2R	TCACTCCTGAGTCTCCATG	ILF2 gene amplification
3F	CCGGAATTCGGATGAGGGGGGACAGAG	pCMV-HA-ILF2/pCMV-Myc-ILF2 construction
3R	CGGGGTACCTCACTCCTGAGTCTCCATG	pCMV-HA-ILF2/pCMV-Myc-ILF2 construction
4F	AGCTTTGTTTAAACACCATGAGGGGGGACAGAG	pWPXL-ILF2 construction
4R	CGACGCGTAACTCCTGAGTCTCCATG	pWPXL-ILF2 construction
5F	CGCGTCGACGGCCGGAAAGAGAGCAAGGA	pCMV-HA-nsp2 construction
5R	GAAGATCTTCATCCCCCTGAAGGCTTCGAA	pCMV-HA-nsp2 construction
6F	AGCTTTGTTTAAACACCATGGCCGGAAAGAGAGCAAGG	pWPXL-nsp2N construction
6R	CGCGACGCGTAATGAAGTCGCCTGGGTGTTGGCTAG	pWPXL-nsp2N construction
7F	AGCTTTGTTTAAACACCATGGGCAAGGACTCGGTCCCTCTG	pWPXL-nsp2M construction
7R	CGCGACGCGTAATTGGTCTAAGAGCCTTCCTGC	pWPXL-nsp2M construction
8F	AGCTTTGTTTAAACACCATGGGCAAGGACTCGGTCCCTCTG	pWPXL-nsp2C construction
8R	CGCGACGCGTAATCCCCCTGAAGGCTTCGAAATTTGC	pWPXL-nsp2C construction

ISSN: 1743-422X