Primersa | Sequence (5′-3′)b | Use |
---|---|---|
1F | AGCTTTGTTTAAACCCATGTACCCATACGATGTTC | pWPXL-nsp9 construction |
1R | CGACGCGTAACTCATGATTGGACCTGAG | |
2F | ATGAGGGGGGACAGAGGCCGTG | ILF2 gene amplification |
2R | TCACTCCTGAGTCTCCATG | |
3F | CCGGAATTCGGATGAGGGGGGACAGAG | pCMV-HA-ILF2/pCMV-Myc-ILF2 construction |
3R | CGGGGTACCTCACTCCTGAGTCTCCATG | |
4F | AGCTTTGTTTAAACACCATGAGGGGGGACAGAG | pWPXL-ILF2 construction |
4R | CGACGCGTAACTCCTGAGTCTCCATG | |
5F | CGCGTCGACGGCCGGAAAGAGAGCAAGGA | pCMV-HA-nsp2 construction |
5R | GAAGATCTTCATCCCCCTGAAGGCTTCGAA | |
6F | AGCTTTGTTTAAACACCATGGCCGGAAAGAGAGCAAGG | pWPXL-nsp2N construction |
6R | CGCGACGCGTAATGAAGTCGCCTGGGTGTTGGCTAG | |
7F | AGCTTTGTTTAAACACCATGGGCAAGGACTCGGTCCCTCTG | pWPXL-nsp2M construction |
7R | CGCGACGCGTAATTGGTCTAAGAGCCTTCCTGC | |
8F | AGCTTTGTTTAAACACCATGGGCAAGGACTCGGTCCCTCTG | pWPXL-nsp2C construction |
8R | CGCGACGCGTAATCCCCCTGAAGGCTTCGAAATTTGC |