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Fig. 4 | Virology Journal

Fig. 4

From: Dual function of the nuclear export signal of the Borna disease virus nucleoprotein in nuclear export activity and binding to viral phosphoprotein

Fig. 4

Characterization of rBoDV P/M-GFP-L131A. a. Expression levels of viral proteins in infected cells. Western blotting was performed with anti-BoDV-N HN132 monoclonal, anti-BoDV-P polyclonal, BoDV-X polyclonal, and anti-β-tubulin monoclonal (Sigma-Aldrich) antibodies. Expression levels of genomic RNA (b) and viral mRNA (c) in infected cells. Total RNA was extracted from infected cells and subjected to RT-qPCR. The experiments were performed independently three times. The data were analyzed with Student’s t-test. Bars show mean ± SD. d. Growth kinetics of rBoDV P/M-GFP-L131A. OL cells were infected with rBoDV P/M-GFP-L131A (L131A) or rBoDV P/M-GFP (WT) at a multiplicity of infection of 1. At days 3, 15, 20, 30, 40, and 50 post-infection, GFP-positive cells were counted using a Tali Image-Based Cytometer (Thermo Fisher Scientific). The experiments were performed independently three times. Bars show mean ± SD

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