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Fig. 3 | Virology Journal

Fig. 3

From: Dual function of the nuclear export signal of the Borna disease virus nucleoprotein in nuclear export activity and binding to viral phosphoprotein

Fig. 3

Interaction of BoDV-N NES mutants with BoDV-P. a. 293 T cells were transfected with pC-FLAG-BoDV-N, pC-BoDV-L, and pCNX2-HA-BoDV-P. At 48 h post-transfection, the cells were subjected to an IP assay using anti-FLAG M2 monoclonal antibody. The IP product was analyzed by western blotting using anti-FLAG M2 polyclonal and anti-HA monoclonal antibodies. b. Nuclear localization of BoDV-Np40 containing L131A mutation. OL cells were transfected with BoDV-Np40- or BoDV-Np40-L131-expression plasmids. At 48 h post-transfection, the cells were subjected to IFA using an anti-BoDV-N HN132 monoclonal antibody, and the percentages of cells expressing N only in the nucleus were calculated. A total of 150 cells were counted in 10 fields. The experiments were performed independently three times. The data were analyzed with Student’s t-test. Bars show mean ± standard deviation (SD) (*; p value <0.05)

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