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Fig. 2 | Virology Journal

Fig. 2

From: Dual function of the nuclear export signal of the Borna disease virus nucleoprotein in nuclear export activity and binding to viral phosphoprotein

Fig. 2

Subcellular localization of BoDV-N NES mutants. a. 293 T cells were transfected with pC-BoDV-MG-Gluc, pC-BoDV-N, pC-BoDV-L, and pCXN2-P. b. 293 T cells were transfected with pC-BoDV-MG-Gluc, pC-BoDV-L, pCXN2-P and 87 ng of pC-BoDV-N (WT) or 2.1 μg of pC-BoDV-N-NES-A (ALL). a and b. At 48 h post-transfection, the cells were subjected to biochemical fractionation. BoDV-N and the fraction marker proteins (snRNP70 for the nucleus and β-tubulin or GAPDH for the cytoplasm) were detected by western blotting using anti-BoDV-N HN132 monoclonal antibody and anti-snRNP70 polyclonal, anti-β-tubulin, or anti-GAPDH monoclonal antibodies, respectively

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