Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

Table 3 Extraction efficiency of the innuPREP MP basic kit on spiked samples (n = 24) were tested by real-time RPA assay, CaPV RPA LFD assay and CaPV real-time qPCR assay respectively

Sample name	Real-time qPCR (CT)	real-time RPA(min)	RPA LFD
liver 1	22	3.6	+
liver 2	27	4.3	+
liver 3	26	4	+
lung 1	31	7.6	+
lung 2	29	7.3	+
lung 3	24	4	+
stomach 1	26	4.3	+
stomach 2	28	4.3	+
stomach 3	32	8.3	+
kidney 1	22	4	+
kidney 2	25	4.6	+
kidney 3	29	7.3	+
lymphatic nodes 1	30	8	+
lymphatic nodes 2	21	3.3	+
lymphatic nodes 3	23	3.6	+
spleen 1	24	4.3	+
spleen 2	26	5	+
spleen 3	29	7.6	+
skin 1	22	4	+
skin 1	27	6.3	+
skin 1	28	6.6	+
nasal swab 1	25	6	+
nasal swab 2	26	6.3	+
nasal swab 3	28	7	+

ISSN: 1743-422X