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Table 3 Extraction efficiency of the innuPREP MP basic kit on spiked samples (n = 24) were tested by real-time RPA assay, CaPV RPA LFD assay and CaPV real-time qPCR assay respectively

From: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

Sample name Real-time qPCR (CT) real-time RPA(min) RPA LFD
liver 1 22 3.6 +
liver 2 27 4.3 +
liver 3 26 4 +
lung 1 31 7.6 +
lung 2 29 7.3 +
lung 3 24 4 +
stomach 1 26 4.3 +
stomach 2 28 4.3 +
stomach 3 32 8.3 +
kidney 1 22 4 +
kidney 2 25 4.6 +
kidney 3 29 7.3 +
lymphatic nodes 1 30 8 +
lymphatic nodes 2 21 3.3 +
lymphatic nodes 3 23 3.6 +
spleen 1 24 4.3 +
spleen 2 26 5 +
spleen 3 29 7.6 +
skin 1 22 4 +
skin 1 27 6.3 +
skin 1 28 6.6 +
nasal swab 1 25 6 +
nasal swab 2 26 6.3 +
nasal swab 3 28 7 +