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Table 2 The specificity of CaPV real-time RPA assay and CaPV RPA LFD assay

From: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

Virus family

Virus specie

Virus strain

Real-time RPA

RPA LFD

Real-time qPCR

Poxviridae

Capripox

GTPV AV40

4.6 min

pos

19(CT)

GTPV AV41

4.6 min

pos

20(CT)

GTPV GS-V1

5 min

pos

20(CT)

Capripox

SPPV Gulang2009

5.3 min

pos

21(CT)

SPPV Jingtai2011

5 min

pos

21(CT)

SPPV Hubei

5.3 min

pos

22(CT)

Poxviridae

ORFV

ORFV/Vaccine/CHA

neg

neg

neg

ORFV

ORFV/HB/CHA

neg

neg

neg

Paramyxovirinae

PPRV

Nigeria 75/1

neg

neg

neg

Picornaviridae

FMDV

FMDV/O/CHA

neg

neg

neg

 

FMDV

FMDV/A/CHA

neg

neg

neg

  1. pos positive, neg negative, GTPV goat pox virus, SPPV Sheep pox virus, ORFV Orf virus, PPRV peste des petits ruminants virus, FMDV foot-and-mouth disease virus