Skip to main content

Advertisement

Table 1 Groups of mice immunized with vaccines by intramuscular and oral route

From: Comparison of the protective efficacy between single and combination of recombinant adenoviruses expressing complete and truncated glycoprotein, and nucleoprotein of the pathogenic street rabies virus in mice

IM groupa 1 2 3 4 5
Vaccineb Ad-0910G Ad-0910G + Ad-0910 N Ad-0910 Gsped Ad-0910 Gsped + Ad-0910 N PBS
Vaccine volume 0.2 mL 0.2 mL (0.1 + 0.1 mL) 0.2 mL 0.2 mL (0.1 + 0.1 mL) 0.2 mL
No. of vaccination 1 1 1 1 1
Challenge routec IM IC IM IC IM IC IM IC IM IC
Oral groupd 6 7 8 9 10
Vaccinee V-RG Ad-0910G + Ad-0910 N V-RG Ad-0910G + Ad-0910 N PBS
Vaccine volume 0.1 mL 0.1 mL (0.05 + 0.05 mL) 0.1 mL 0.1 mL (0.05 + 0.05 mL) 0.1 mL
No. of vaccinationf 1 1 2 2 1
Challenge routeg IC IC IC IC IC
  1. aFifty 4-week-old mice were randomly grouped into 5 groups (ten/group) for intramuscular (IM) vaccination
  2. bThe titers of Ad-0910G, Ad-0910Gsped and Ad-0910 N were 108.0 TCID50/mL, 108.0 TCID50/ mL and 107.7 TCID50/mL, respectively
  3. cImmunized mice of each group were divided into two subgroup and intramuscularly (IM) and intracranially (IC) challenged with CVS-N2c at 21 days following inoculation of recombinant adenoviruses
  4. dThirty 4-week-old mice were divided into 4 groups (five/group) and control group (ten/group) for oral vaccination
  5. eThe titers of V-RG (Raboral V-RG®, Merial), Ad-0910G and Ad-0910 N were 107.8 TCID50/mL, 108.0 TCID50/mL and 107.7 TCID50/mL, respectively
  6. fMice in the groups 6 and 7 were vaccinated with 0.1 ml of V-RG vaccine or mixed virus (Ad-0910G + Ad-0910 N) via the oral route only once. Mice in the groups 8 and 9 were vaccinated orally once again a week after initial vaccination
  7. gAll mice were challenged with CVS-N2c by the IC route 14 days post-vaccination