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Fig. 2 | Virology Journal

Fig. 2

From: Comparison of the protective efficacy between single and combination of recombinant adenoviruses expressing complete and truncated glycoprotein, and nucleoprotein of the pathogenic street rabies virus in mice

Fig. 2

Immunofluorescence assay (IFA) and Western blot analysis for identification of G and N proteins expressed from recombinant adenoviruses. The IFA was performed in mock-infected 293A cells (a) and cells infected with recombinant adenoviruses Ad-0910 N (b), Ad-0910G (c), and Ad-0910Gsped (d). Cells were fixed 2 days post-infection and subjected to antibody staining using RABV G and N protein-specific antibodies. Specific fluorescence (arrows) was detected in 293A cells infected with each recombinant adenovirus. Bars, 100 μm. Western blot analysis was conducted using 293A cell lysates infected with Ad-0910G, Ad-0910Gsped and Ad-0910 N, and monoclonal antibodies against the G or N protein of RABV. (e) Lane 1, 293A cell lysates infected with Ad-0910G; lane 2, 293A cell lysates infected with Ad-0910Gsped; lanes 3 and 4, NG108–15 cell lysates infected with the ERA strain; lane 5, mock-infected 293A cell lysates, (f) lane 1, 293A cell lysates infected with Ad-0910 N; lane 2 NG108–15 cell lysates infected with the ERA strain; lane 3, mock-infected 293A cell lysates

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