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Table 1 Real-time RT PCR of amniotic/allantoic fluids (AF) and chorioallantoic membranes (CAM) from embryonated chicken eggs inoculated with A/equine/Pulawy/2008

From: Chorioallantoic membranes of embryonated chicken eggs as an alternative system for isolation of equine influenza virus

Dilution

10−5

10−6

10−7

10−8

Sample

AF

CAM

AF

CAM

AF

CAM

AF

CAM

Ct (n = 6)

19.3a

11.3

36

27.4

40

32.1

40

29.7

19.3b

12.3

18.4a

8.5

40

32

40

34.6

19.4a

12.2

19a

13.3

40

32.7

40

33.1

14.5b

12.6

35.6

24.9

40

30.1

40

34.9

16.3b

12.4

18.2a

8.9

40

33.9

38.9

34.2

19.5a

11.5

35.9

24.3

40

30.8

40

32.4

Mean

18.05

12.05

27.18

17.88

40

31.93

39.81

33.15

SD

2.1

0.5

9.5

8.6

0

1.4

0.4

1.9

p

 

0.0050

 

0.1735

 

0.0028

 

0.0037

  1. aHA 64
  2. bHA 128
  3. SD standard deviation
  4. p - p value calculated with Mann–Whitney U test