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Fig. 7 | Virology Journal

Fig. 7

From: Epitope mapping and characterization of a novel Nsp10-specific monoclonal antibody that differentiates genotype 2 PRRSV from genotype 1 PRRSV

Fig. 7

Reactivity of mAb 4D9 with the mutant of the epitope 286AIQPDYRDKL295. The plasmid pCMV-HA-E-1 expressing the epitope (E-1) and pCMV-HA-E-1-mutant expressing a mutant of E-1 with 2 mutations (290D/C290, 293D/E293) were constructed and transfected into Vero cells. The reaction of mAb 4D9 with the two epitopes was tested. a Western blotting of lysates of Vero cells transfected with pCMV-HA-E-1or pCMV-HA- E-1-mutant. b Confocal microscopy of Vero cells transfected with pCMV-HA-E-1 or pCMV-HA-E-1-mutant

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