Fig. 5From: Epitope mapping and characterization of a novel Nsp10-specific monoclonal antibody that differentiates genotype 2 PRRSV from genotype 1 PRRSVFine mapping of the mAb 4D9 epitope in Nsp10. Recombinant plasmids expressing a series of truncated Nsp10 were transfected into 293FT cells, and the expressed peptides were probed with anti-GFP polyclonal antibodies and the mAb 4D9 by Western blottingBack to article page