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Fig. 1 | Virology Journal

Fig. 1

From: Epitope mapping and characterization of a novel Nsp10-specific monoclonal antibody that differentiates genotype 2 PRRSV from genotype 1 PRRSV

Fig. 1

Analysis of expressed recombinant Nsp10 by SDS-PAGE (a) and Western blotting (b) with anti-His mAb. Lane M: protein molecular weight marker; Lane 1: lysates of pET-28a-Nsp10 transformed E. coli BL21 (DE3) before IPTG induction; Lane 2: lysates of pET-28a-Nsp10 transformed E. coli BL21 (DE3) after IPTG induction; Lane 3 and 4: purified recombinant Nsp10; Lane 5: lysates of pET-28a transformed E. coli BL21 (DE3) as negative control

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