Fig. 5

The inhibitory effect of of CypB specific siRNAs on ORFV replication in MDBK cells. a At 48 h post siRNA transfection, the siRNA-treated cells were infected with ORFV-Jilin, respectively. Viral DNA was extracted from cell cultures at 12, 24, 36, 48, 60 and 72 h postinfection. The qPCR method based on the DNA polymerase gene of ORFV was applied to determine the viral DNA copy number. b Viral yields were quantitated in the siRNA-treated cells by TCID₅₀ assay. TCID₅₀ values are the means of three repeat titrations at the same points indicated. Statistical analysis was performed using one-way ANOVA followed by Fisher’s LSD test. Values are the means ± SEM. (** P < 0.01, *** P < 0.001)