Virology Journal

Table 3 Predicted cleavage sites in the polyproteins of T’Ho virus and select other flaviviruses

From: Complete genome sequence of T’Ho virus, a novel putative flavivirus from the Yucatan Peninsula of Mexico

Virus	Junction
	¹VirC/Anch	²C/prM	³pr/M	²prM/E	²E/NS1	⁴NS1/NS2A
T’Ho virus	KNNKR↓GMTTT	ATTMA↓ARLSS	RRGRR↓SINIA	VPAYS↓LNCLG	MNVHA↓DTGCV	SMVVA↓GHGSS
ROCV	RKAKR↓GNGSV	TGSMA↓LRLGT	RRGRR↓SVNIP	APAYS↓INCLG	MNVHA↓DTGCA	SKVTA↓GTGND
ILHV	KEKKK↓SFSTA	TAVAG↓LKISS	RRGRR↓AINIP	APAYS↓LNCLG	VNVHA↓DTGCA	SKVSA↓GNGQT
BAGV	GKKKR↓GGTTV	GVAQA↓IKIGS	RRSRR↓SITVH	APAYS↓FNCLG	TNVHA↓DTGCA	SRVTA↓YDGAG
WNV	KQKKR↓GGTAG	ACAGA↓VTLSN	RRSRR↓SLTVQ	APAYS↓FNCLG	VNVHA↓DTGCA	SRVNA↓YNADM
	¹NS2A/NS2B	¹NS2B/NS3	¹NS3/NS4A	¹NS4A/2K	²2K/NS4B	¹NS4B/NS5
T’Ho virus	GMTRR↓GWPAS	RLQKR↓GGVLW	AAGKR↓SASAI	PEKQR↓SQTDN	SAVSA↓NEMGW	PKVKR↓GGGTG
ROCV	CATKR↓GWPAS	KIHKR↓GGVLW	AAGKR↓SAGSM	PEKQR↓SQTDN	SAVSA↓NEMGW	PKVKR↓GGIAA
ILHV	SLGKR↓GWPAS	KIHKR↓GGVMW	AAGKR↓SAGSV	PEKQR↓SQTDN	GAVSA↓NEMGW	PKLKR↓GGGSA
BAGV	PSNRR↓GWPVS	HSPKR↓SGAIW	ACGKR↓SAIGV	PEKQR↓SQTDS	GTVAS↓NEMGW	GSMRR↓GGGKG
WNV	PNRKR↓GWPAT	QYTKR↓GGVLW	ASGKR↓SQIGL	PEKQR↓SQTDN	GAVAA↓NEMGW	PGLKR↓GGAKG

Predicted cleavage sites for ROCV, WNV and BAGV were identified in earlier studies ([8]; [6]; [27]; [32]). Most of the predicted cleavage sites for ILHV are described in the corresponding Genbank entry (accession no. AAV34155) and the remainder determined here. Underlined sequences have been experimentally verified. Cleavage events are mediated by ¹the viral NS2B/NS3 serine protease ([6]; [7]; [5]), ²a host signal peptidase [6], ³the cellular furin protease ([6]; [35]; [41]) and ⁴a membrane-bound host protease in the endoplasmic reticulum [15].

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ISSN: 1743-422X

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