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Table 3 Predicted cleavage sites in the polyproteins of T’Ho virus and select other flaviviruses

From: Complete genome sequence of T’Ho virus, a novel putative flavivirus from the Yucatan Peninsula of Mexico

Virus Junction
  1VirC/Anch 2C/prM 3pr/M 2prM/E 2E/NS1 4NS1/NS2A
T’Ho virus KNNKR↓GMTTT ATTMA↓ARLSS RRGRR↓SINIA VPAYS↓LNCLG MNVHA↓DTGCV SMVVA↓GHGSS
ROCV RKAKR↓GNGSV TGSMA↓LRLGT RRGRR↓SVNIP APAYS↓INCLG MNVHA↓DTGCA SKVTA↓GTGND
ILHV KEKKK↓SFSTA TAVAG↓LKISS RRGRR↓AINIP APAYS↓LNCLG VNVHA↓DTGCA SKVSA↓GNGQT
BAGV GKKKR↓GGTTV GVAQA↓IKIGS RRSRR↓SITVH APAYS↓FNCLG TNVHA↓DTGCA SRVTA↓YDGAG
WNV KQKKR↓GGTAG ACAGAVTLSN RRSRR↓SLTVQ APAYS↓FNCLG VNVHA↓DTGCA SRVNA↓YNADM
  1NS2A/NS2B 1NS2B/NS3 1NS3/NS4A 1NS4A/2K 22K/NS4B 1NS4B/NS5
T’Ho virus GMTRR↓GWPAS RLQKR↓GGVLW AAGKR↓SASAI PEKQR↓SQTDN SAVSA↓NEMGW PKVKR↓GGGTG
ROCV CATKR↓GWPAS KIHKR↓GGVLW AAGKR↓SAGSM PEKQR↓SQTDN SAVSA↓NEMGW PKVKR↓GGIAA
ILHV SLGKR↓GWPAS KIHKR↓GGVMW AAGKR↓SAGSV PEKQR↓SQTDN GAVSA↓NEMGW PKLKR↓GGGSA
BAGV PSNRR↓GWPVS HSPKR↓SGAIW ACGKR↓SAIGV PEKQR↓SQTDS GTVAS↓NEMGW GSMRR↓GGGKG
WNV PNRKR↓GWPAT QYTKR↓GGVLW ASGKR↓SQIGL PEKQR↓SQTDN GAVAA↓NEMGW PGLKR↓GGAKG
  1. Predicted cleavage sites for ROCV, WNV and BAGV were identified in earlier studies ([8]; [6]; [27]; [32]). Most of the predicted cleavage sites for ILHV are described in the corresponding Genbank entry (accession no. AAV34155) and the remainder determined here. Underlined sequences have been experimentally verified. Cleavage events are mediated by 1the viral NS2B/NS3 serine protease ([6]; [7]; [5]), 2a host signal peptidase [6], 3the cellular furin protease ([6]; [35]; [41]) and 4a membrane-bound host protease in the endoplasmic reticulum [15].