|
Primer set
|
Target
|
Primer
|
Sequence (5′ ➔ 3′)
|
Amplicon (bp)
|
|---|
|
A
|
IBV 1a – IBV 1a
|
IBV.F02
|
GGTGTAACACCAGAGATAAATG
|
1416
|
| | |
IBV.R02
|
ATTTACGACGTCAAGAGCGTC
| |
|
B
|
MHV 1b – MHV S
|
1173
|
GACTTAGTCCTCTCCTTGATTG
|
2479
|
| | |
1127
|
CCAGTAAGCAATAATGTGG
| |
|
C
|
IBV 1b – IBV S
|
IBV.F73
|
TCAGCATGGACGTGTGGTTA
|
992
|
| | |
IBV.R73
|
CCCCATGTAAATGCCAACCA
| |
|
D
|
IBV S – IBV S
|
IBV.F14
|
TAAATGGTGATCTTGTTT
|
708
|
| | |
IBV.R13
|
CGCTCTTAGTAACATAAAC
| |
|
E
|
IBV S – IBV M
|
IBV.F15
|
TGCTGCTTCCTTTAATAAG
|
1994
|
| | |
IBV.R15
|
CTGCGACAAGACCTCCTG
| |
|
F
|
IBV 1b – MHV S
|
mIBV.F47
|
TCAGCATGGACGTGTGGTTA
|
1035
|
| | |
mIBV.R47
|
CCCAGGCCTTGTGAAACTTC
| |
|
G
|
MHV S – MHV S
|
MHV.F05
|
ACCCTCCGCTACTACGTTTT
|
966
|
| | |
MHV.R05
|
AGGCAGGTATCATGTGACCA
| |
|
H
|
MHV S – IBV M
|
MHV.F08
|
GGATGGGTTTGATGCAACCA
|
2129
|
| | |
IBV.R37
|
GAGAAAGCACCATTGGCACA
| |
- Primer set letters refer to Figs. 1 and 3
