Table 3 Primer sets used for genetic characterization of mIBV and rIBV-wt
Primer set | Target | Primer | Sequence (5′ ➔ 3′) | Amplicon (bp) |
|---|---|---|---|---|
A | IBV 1a – IBV 1a | IBV.F02 | GGTGTAACACCAGAGATAAATG | 1416 |
IBV.R02 | ATTTACGACGTCAAGAGCGTC | |||
B | MHV 1b – MHV S | 1173 | GACTTAGTCCTCTCCTTGATTG | 2479 |
1127 | CCAGTAAGCAATAATGTGG | |||
C | IBV 1b – IBV S | IBV.F73 | TCAGCATGGACGTGTGGTTA | 992 |
IBV.R73 | CCCCATGTAAATGCCAACCA | |||
D | IBV S – IBV S | IBV.F14 | TAAATGGTGATCTTGTTT | 708 |
IBV.R13 | CGCTCTTAGTAACATAAAC | |||
E | IBV S – IBV M | IBV.F15 | TGCTGCTTCCTTTAATAAG | 1994 |
IBV.R15 | CTGCGACAAGACCTCCTG | |||
F | IBV 1b – MHV S | mIBV.F47 | TCAGCATGGACGTGTGGTTA | 1035 |
mIBV.R47 | CCCAGGCCTTGTGAAACTTC | |||
G | MHV S – MHV S | MHV.F05 | ACCCTCCGCTACTACGTTTT | 966 |
MHV.R05 | AGGCAGGTATCATGTGACCA | |||
H | MHV S – IBV M | MHV.F08 | GGATGGGTTTGATGCAACCA | 2129 |
IBV.R37 | GAGAAAGCACCATTGGCACA |