Primer
|
Sequence (5′ ➔ 3′)
|
Function
|
---|
XhoI-t-MHV-S.F01
|
CTCGAG
TTATATTGGTGATTTTAGATGTATCCAG
|
Cloning MHV S
|
MHV-S-StyI-XhoI.R01
|
CTCGAGCCAAGGCCATTTCACATACATTTC
|
Cloning MHV S
|
p-MHV-S-SDM414.F02
|
AGCTTGTGAACTCAAACGGTG
|
SDM EcoRI
|
p-MHV-S-SDM414.R02
|
GGATACATCTAAAATCACCAATATAAC
|
SDM EcoRI
|
p-MHV-S-SDM2823.F03
|
TTACTATAAGTTCGAGACTGCC
|
SDM XhoI
|
p-MHV-S-SDM2823.R03
|
CACCCTGCATTAATGCAC
|
SDM XhoI
|
IBV-H52_N_ATG_FW
|
ACCATGGCGAGCGGTAAGA
|
N-transcript
|
IBV-M41-#2-IR-RV
|
TTTTTTTTTTTTTTTTTTTGCTCTAACTCTATACTAG
|
N-transcript
|
-
XhoI restriction enzyme sites in sequences of primers XhoI-t-MHV-S.F01 and MHV-S-StyI-XhoI.R01 are in italics; an additional thymidine residue to keep the MHV spike gene ectodomain sequence in frame with the IBV spike gene signal sequence is in bold. SDM = site directed mutagenesis