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Fig. 4 | Virology Journal

Fig. 4

From: A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination

Fig. 4

In ovo characteristics of IBV and rIBV-wt. a Growth kinetics of IBV and rIBV-wt were assessed by quantitative RT-qPCR analysis of RNA extracted from allantoic fluid of inoculated embryonated eggs collected at 6, 12, 24, 36 and 48 hpi. Data points represent means and standard deviations of 5 eggs per condition, with all samples run and analyzed in triplicates, using a tenfold dilution series of IBV H52 BI as reference for quantification. b Embryonic death is indicated as a percentage of all remaining animals at each time point

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