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Fig. 3 | Virology Journal

Fig. 3

From: A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination

Fig. 3

Genetic characterization of wild type viruses, recombinant viruses and donor plasmids. PCR was performed on cDNA templates of viral RNA extracted from infected LR7 cell culture supernatants (MHV and mIBV) and allantoic fluid of inoculated embryonated eggs (IBV and rIBV-wt), plasmid DNA (p-mIBV and p-IBV), or no template control (−).Primer set letters A to H correspond with letters depicted in Fig. 1; detailed information on the primers is given in Table 3

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