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Fig. 5 | Virology Journal

Fig. 5

From: Iron availability affects West Nile virus infection in its mosquito vector

Fig. 5

Iron chelator, deferoxamine, reduces viral titer in Culex cells

Culex (Hsu) cells were infected with WNV with/without deferoxamin (DFX, 10 uM). As a control cells were either uninfected and/or treated with DMSO (diluent). Total RNA and media supernatant were collected 48 hpi. Real time RT-PCR was performed using ferritin (heavy chain) (a), NRAMP (b) and WNV NS1 (c) primers. RpL32 primers were used as an internal control. Error bars represent standard errors from means of three separate experiments with assays performed in triplicate (Student’s t-test *p < 0.05, comparing with control cells). d Viral titer estimation by plaque assays conducted on the supernatant media collected from cells treated as in (a). (Student’s t-test *p < 0.05, comparing with control infected cells). e Cell viability was measured from Culex (Hsu) cells treated with different concentrations (0, 10, 100 and 1000 uM) of DFX for 48 h using trypan blue exclusion

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