Reducing intracellular iron affects viral titers.
Culex (Hsu) cells were transfected with dsRNA against Culex NRAMP 24 h before infection with WNV (MOI of 1). GFP-dsRNA was used as a negative control. Cells were also treated with FAC (100 uM). Total RNA and media supernatant were collected 48 hpi. Real time RT-PCR was performed using NRAMP (a) and ferritin (heavy chain) (b) primers. RpL32 primers were used as an internal control. Error bars represent standard errors from means of three separate experiments with assays performed in triplicate (Student’s t-test *p < 0.05, comparing with GFP-dsRNA cells). c Viral titer estimation by plaque assays conducted on the supernatant media collected from cells treated as in (a). (Student’s t-test *p < 0.05, comparing with control infected (GFP-dsRNA) cells)