Table 1 List of primers used for PCR amplification of group C rotavirus Group C Rotavirus positive controls were provided by Dr. Baoming Jiang, Gastroenteritis & Respiratory Viruses Lab, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta
Gene | Primer (Polarity) | Sequence (5′–3′) | Position | Reference |
|---|---|---|---|---|
VP7 | VP7 FP-1 BMJ-13 | 5′-GGC ATT TAA AAA AGA AGA AGC TG-3′ 5′-AGC CAC ATG ATC TTG TTT -3′ | 1–23 | Jiang et al.,1995 |
1063–1046 | ||||
BMJ-107 BMJ-13 | 5′ TGT TTG GAG ATG TGA TGA -3′ 5′-AGC CAC ATG ATC TTG TTT-3′ | 546–563 | Jiang et al.,1995 | |
1063–1046 | ||||
VP6 | VP6 FP-1 BMJ-44 | 5′- GCA TTT AAA ATC TCA TTC ACA A-3′ 5′-AGC CAC ATA GTT CAC ATT TCA-3′ | 2–22 | Jiang et al.,1995 |
1353–1333 | ||||
BMJ-145 BMJ-144 | 5′-AGT CCG TTC TAT GTG ATT C-3′ 5′-CCT TCT GGG GAT CAT CCA T-3′ | 1014–1032 | Jiang et al.,1995 | |
1331–1313 | ||||
VP4 | GCVP4-1FP GCVP4-12RP | 5′- GGC TTA AAA AAT AGA GAT CGA TGG CG -3′ 5′- CAT AAA CAA GTT GCA ACC TTG ATG -3′ | 1–26 | Yamamoto et al., 2011 |
1275–1252 | ||||
GCVP4-2FP GCVP4-12RP | 5′- GTA AGG ACT CAT TGT GGC AAG A -3′ 5′- CAT AAA CAA GTT GCA ACC TTG ATG -3′ | 843–820 | Yamamoto et al., 2011 | |
1275–1252 |